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axio observer inverted wide field microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio observer inverted wide field microscope
    Axio Observer Inverted Wide Field Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4847 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wide+field+microscope/Inverted+microscope+Axio+Observer+3/us12577530-675-7-6
    Average 99 stars, based on 4847 article reviews
    axio observer inverted wide field microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Increased vascular smooth muscle cell senescence in aneurysmal Fibulin-4 mutant mice
    Article Snippet: Slides were mounted with Vectashield containing DAPI (H-1200, Vector laboratories) and sealed with nail polish. .. Images were recorded on a near-infrared wide-field microscope (Axio Imager D2, Zeiss) using a 10× objective lens. .. Quantification of the number of nuclei in αSMA-positive area was performed using Fiji (ImageJ) .

    Article Title: The ESCRT machinery regulates retromer-dependent transcytosis of septate junction components in Drosophila
    Article Snippet: .. Images were acquired on a Zeiss AxioImager Z1 wide field microscope immediately after mounting (within a timeframe of 5 min). ..

    Article Title: A REM-active basal ganglia circuit that regulates anxiety.
    Article Snippet: Before mounting, slices were incubated with Hoechst 33342 (Thermo Fisher Scientific) for 15min followed by rinsing with PBS. .. Finally, slices were mounted on slides, embedded in Dako mounting medium (Agilent Technologies) and imaged using an inverted wide-field microscope (Zeiss Axio Observer) or a Leica SP5 MP confocal microscope (Facility for Imaging by Light Microscopy, FILM, Imperial College London). .. Primary antibodies usedwere rabbit polyclonal cFos (1:4000, Sigma-Aldrich); rat monoclonal mCherry (1:2000, Thermo Fisher Scientific); chicken polyclonal GFP (1:1000, Abcam); and rabbit polyclonal somatostatin (1:100, Abcam); mouse monoclonal tyrosine hydroxylase (1:1000, Merck), secondary antibodies were Alexa Fluor 488 goat anti-rabbit, Alexa Fluor 488 goat anti-mouse, Alexa Fluor 594 goat anti-rabbit, Alexa Fluor 594 goat anti-mouse (1:1000, Invitrogen Molecular Probes, UK).

    Article Title: Increased vascular smooth muscle cell senescence in aneurysmal Fibulin-4 mutant mice
    Article Snippet: After incubation, the coverslips were washed with PBS + 0.1% Triton X-100 and shortly washed with PBS, after which they were mounted on glass slides with Vectashield containing DAPI (H-1200, Vector laboratories) and sealed with nail polish. .. Images were recorded on a near-infrared wide-field microscope (Axio Imager D2, Zeiss) using a 20× objective lens. .. Images for quantification of the staining for 53BP1 and γH2AX were recorded on a TCS SP5 confocal microscope (Leica) using a 40× objective lens.

    Article Title: Bacterial exploration of solid/liquid interfaces: developing platforms to control the physicochemical microenvironment.
    Article Snippet: Bacterial long-term contamination of surfaces is a promiscuous phenomenon often linked to harmful processes.. Early bacterial exploration of interfaces, governed by adhesion and individual motility, is a known determinant of the subsequent development and persistence of bacterial colonies.. However, the mechanisms by which bacteria integrate various environmental signals at these interfaces and modulate their behavior in response remain poorly understood.

    Article Title: The regulation of vacuole morphology in stigma papilla cells is involved in water transfer to pollen in Arabidopsis thaliana
    Article Snippet: .. Pollen was placed onto a papilla cell from the mounted anthers using a micromanipulator (MMO-4, Narishige) and photographed at 1-min intervals for 15 min following the initiation of pollination using a wide-field microscope (Axio Observer 7, Zeiss) with a 20 × objective (Zeiss). ..

    Article Title: CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrity
    Article Snippet: For live imaging, zebrafish embryos were mounted in an uncoated 8-well μ-slide (#80827) (Ibidi) in 1.5% low melting point agarose dissolved in egg water (60 μg/mL sea salts (Sigma-Aldrich; S9883) in MilliQ) with addition of 0.02% (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine) (#A5040) (Sigma-Aldrich). .. Zebrafish embryos were imaged after 20 minutes using a Zeiss wide field microscope at 10x magnification. ..

    Article Title: A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over
    Article Snippet: .. Images of z-stacks at 0.2μm intervals were acquired using a 100X objective lens using a Delta Vision Deconvolution system equipped with an Olympus 1X70 microscope or a 63X objective lens of a wide-field microscope (Zeiss Axio Observer inverted microscope platform). ..

    Imaging:

    Article Title: A REM-active basal ganglia circuit that regulates anxiety.
    Article Snippet: Before mounting, slices were incubated with Hoechst 33342 (Thermo Fisher Scientific) for 15min followed by rinsing with PBS. .. Finally, slices were mounted on slides, embedded in Dako mounting medium (Agilent Technologies) and imaged using an inverted wide-field microscope (Zeiss Axio Observer) or a Leica SP5 MP confocal microscope (Facility for Imaging by Light Microscopy, FILM, Imperial College London). .. Primary antibodies usedwere rabbit polyclonal cFos (1:4000, Sigma-Aldrich); rat monoclonal mCherry (1:2000, Thermo Fisher Scientific); chicken polyclonal GFP (1:1000, Abcam); and rabbit polyclonal somatostatin (1:100, Abcam); mouse monoclonal tyrosine hydroxylase (1:1000, Merck), secondary antibodies were Alexa Fluor 488 goat anti-rabbit, Alexa Fluor 488 goat anti-mouse, Alexa Fluor 594 goat anti-rabbit, Alexa Fluor 594 goat anti-mouse (1:1000, Invitrogen Molecular Probes, UK).

    Light Microscopy:

    Article Title: A REM-active basal ganglia circuit that regulates anxiety.
    Article Snippet: Before mounting, slices were incubated with Hoechst 33342 (Thermo Fisher Scientific) for 15min followed by rinsing with PBS. .. Finally, slices were mounted on slides, embedded in Dako mounting medium (Agilent Technologies) and imaged using an inverted wide-field microscope (Zeiss Axio Observer) or a Leica SP5 MP confocal microscope (Facility for Imaging by Light Microscopy, FILM, Imperial College London). .. Primary antibodies usedwere rabbit polyclonal cFos (1:4000, Sigma-Aldrich); rat monoclonal mCherry (1:2000, Thermo Fisher Scientific); chicken polyclonal GFP (1:1000, Abcam); and rabbit polyclonal somatostatin (1:100, Abcam); mouse monoclonal tyrosine hydroxylase (1:1000, Merck), secondary antibodies were Alexa Fluor 488 goat anti-rabbit, Alexa Fluor 488 goat anti-mouse, Alexa Fluor 594 goat anti-rabbit, Alexa Fluor 594 goat anti-mouse (1:1000, Invitrogen Molecular Probes, UK).

    Inverted Microscopy:

    Article Title: A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over
    Article Snippet: .. Images of z-stacks at 0.2μm intervals were acquired using a 100X objective lens using a Delta Vision Deconvolution system equipped with an Olympus 1X70 microscope or a 63X objective lens of a wide-field microscope (Zeiss Axio Observer inverted microscope platform). ..



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    Image Search Results


    Wide-field epifluorescence microscope optical layout. Schematic showing illumination and detection beam paths, component positions, and optical layout. Component specifications detailed in .

    Journal: bioRxiv

    Article Title: Multimodal immobilization of second-instar Drosophila melanogaster larvae using PF-127 hydrogel and diethyl ether for calcium imaging

    doi: 10.64898/2026.03.19.713048

    Figure Lengend Snippet: Wide-field epifluorescence microscope optical layout. Schematic showing illumination and detection beam paths, component positions, and optical layout. Component specifications detailed in .

    Article Snippet: All images were acquired using a custom-built wide-field epifluorescence microscope ( ) constructed on a Thorlabs 30 mm cage system with a cage cube as the central junction for excitation and emission paths.

    Techniques: Microscopy